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Qiagen Protocols

Qiaprep Miniprep Handbook: https://drive.google.com/open?id=0B2L1ybPbRflPRERlMkJYd3h0YmM Taq PCR Handbook: https://drive.google.com/open?id=0B2L1ybPbRflPTGZCZ1BnUmVrUEE HiSpeed Plasmid Purification Handbook: https://drive.google.com/open?id=0B2L1ybPbRflPd0tKdmQ0UTBhS3c QIAMP DNA Microkit: https://drive.google.com/open?id=0B2L1ybPbRflPU0lmMHJ5YVRjVWc RNAeasy Mini Kit: https://drive.google.com/open?id=1Kddw79oD4eg_drvZ1YLWnhV12R_eouTa QIAquick Spin Handbook (Gel Extraction): https://drive.google.com/open?id=0B2L1ybPbRflPR1B0MUNjU19ycEk AllPrep DNA/RNA Micro Kit: https://drive.google.com/open?id=1TgI3RQEEtUwh_0YQxOyIpO1K6N-TWuKM QIAGEN Mini, Midi and Maxi Prep Kit: https://drive.google.com/open?id=1sPWSZN8tX7BWN9MvLRD3iqQGrRfOxe23 QIAMP DNA Blood Mini Kit: https://drive.google.com/open?id=1sPWSZN8tX7BWN9MvLRD3iqQGrRfOxe23    

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Murine Protocols

Mouse Tail DNA Isolation: https://drive.google.com/open?id=1I-wSsMwrbGn5wNdd3LKwoB0nfd6z_PJbXmT6nxl6aeI Genotyping Primers and PCR cycles per strain: https://drive.google.com/open?id=0B2e0bKaB-0maeDk3UzlKTUYyejA Spleen RNA isolation: https://drive.google.com/open?id=0B2L1ybPbRflPczZuV1ltWmZXWTQ RBC Lysis Protocol: https://drive.google.com/open?id=0B2L1ybPbRflPTDNnRGhMczZldzg Isolating BM for culture: https://drive.google.com/open?id=0B2L1ybPbRflPU2pmZkpsdnJqajg Isolating and Staining BM and spleens: https://drive.google.com/open?id=0B2L1ybPbRflPZUwtY2tIbElUVzQ CD3+ Selection from Peripheral Blood: https://drive.google.com/open?id=0B2L1ybPbRflPWnZjcmczS254Nm8

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Protocols

Miniprep Protocol: https://drive.google.com/open?id=1gd_Tv3xQT57hpK1XVHyF9JWKUEGX-CC3dU-FGXtvkBo Giemsa Staining Protocol: https://drive.google.com/open?id=0B2L1ybPbRflPQm1sZGctUXZBa1U    

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Ordering/Financials

Ordering Instructions/Tips    Orders should be placed/ Processed twice a week. Usually Mondays and Wednesdays work best, so the administrative assistant can purchase the items on Tuesdays and Thursdays. The lab manager receives an updated spending sheet from our grants…

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